7 factors to consider when isolating specific cell types. Isolating unique cell populations for further experimental analyses can answer critical research questions in clinical research – empowering observational and therapeutic studies. A popular approach is to isolate the heterogeneous population of cells in peripheral blood mononuclear cells (PBMCs) using the Ficoll method (link), followed by further purification to yield unique immune cell subsets, such as activated T cells for further downstream analyses. Specific cell types can also be isolated directly from whole peripheral blood, bone marrow or cord blood.
Big Data in Clinical Research — Sharing, Dissemination, and Repurposing Sanchita Bhattacharya, Bioinformatics Project Lead at Bakar Computational Health Sciences Institute, UCSF School of Medicine
Precision Medicine for Genetic Disease: from Prevention to Treatment Jun Liao, Ph.D., FACMG, Laboratory Director and Assistant Professor @Columbia University Medical Center
TSHR-specific lymphocytes in Ad-TSHR-immunized native and HLA-DR3-transgenic mice and in Graves´ disease patient blood using MHCII tetramers Degen, Gavvovidis, Blankenstein et al Thyroid : The Official journal of the American Thyroid Association (2020)
Isolation of monoclonal antibodies from anti-synthetase syndrome patients and affinity maturation by recombination of independent somatic variants Burman, Chong, Duncan et al mAbs (2020) 12